Figure 5.

H-89 treatment selectively increases secretin receptor-mediated cyclic AMP response in NG108-15 cells. Cells were treated with 10 μM H-89 for 30 min prior to and during addition of either secretin (100 nM), forskolin, (10 μM), NECA (10 μM) or iloprost (1 μM) for 60 min. Data are mean±s.e.mean (bars) from five independent experiments, each performed in triplicate. Cyclic AMP accumulation was expressed as a per cent of the respective control agonist responses. ^*P<0.05, versus respective control, one sample t-test.