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. 2002 Apr;135(8):1927–1934. doi: 10.1038/sj.bjp.0704658

Figure 4.

Figure 4

NX binding kinetics in trophoblasts. (A) Equilibrium binding of [3H]-NX: Binding of [3H]-NX was done by incubating intact trophoblasts with increasing concentrations (0.1 – 5 nM) of [3H]-NX in KRH buffer on ice for 2 h. Nonspecific binding was obtained using 10 μM unlabelled NX, and this was subtracted from total binding. NX binding shows saturable kinetics with a Kd=1.15±0.2 nM and a Bmax=0.43±0.03 pmol (106 cells)−1. The R value (0.995) shows the goodness-of fit. We obtained a Hill coefficient of 0.789 when binding data were fit to either a single-site or a two-site model. (B) Scatchard analysis of NX binding: A linear fit to the Rosenthal transformation of the binding shows a single population of NX binding sites. The R value (0.942) shows the goodness-of fit. The data represent the mean±s.e.m. from two experiments performed in triplicate on different batches of trophoblast cultures.