Figure 3.

Effects of polyunsaturated fatty acids on IL-1β-induced iNOS protein expression in VSMCs. Cells were stimulated with 3 ng ml⁻¹ IL-1β for 24 h in the absence or the presence of each fatty acid as indicated. iNOS expressions were analysed by Western blot. (A) Dose-dependent effect of DHA on iNOS expression. (B) Effects of 30 μM DHA, EPA and arachidonic acid on IL-1β-induced iNOS expression. Control means IL-1β alone. (C) Summary of densitometric analysis expressed as percentage taking the control as 100%. Each column represents mean±s.e.mean of replicate experiments indicated in parentheses. ^**P<0.01 versus control.