Figure 1.

Effects of BAY 58-2667 on sGC activity. (A) Structure of BAY 58-2667. (B) Stimulation of purified sGC by BAY 58-2667 in the absence and presence of DEA/NO (10 and 100 nM) and ODQ (10 μM). The specificity is expressed as fold stimulation versus basal activity in the presence of Mg²⁺: 111 nmol min⁻¹ mg⁻¹ for the purified sGC. (C) Stimulation of haem free sGC by BAY 58-2667 in the absence and presence of ODQ. The specificity of sGC is expressed as fold stimulation versus basal activity in the presence of Mg²⁺: 169 nmol min⁻¹ mg⁻¹ for the haem-free sGC. Each value represents the mean±s.e.mean from three (B) and five (C) independent experiments performed in duplicate. (D) Haem spectra of sGC in the presence of BAY 58-2667 and DEA/NO. These data are representative of three independent determinations.