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. 2002 Aug;136(7):1015–1022. doi: 10.1038/sj.bjp.0704805

Figure 3.

Figure 3

Effects of Y27632, U73122, G12G228A and G13G225A on ET-1-induced actin stress fiber formation in CHO-ETBRΔ403. Cells were stimulated with (B∼F) or without (A) 10 nM ET-1. Y-27632 at 10 μM (C) and U73122 at 5 μM (D) were added 15 min before stimulation with ET-1. Expression plasmids encoding G12G228A (E) and G13G225A (F) were microinjected into the cell nuclei 24 h before stimulation with ET-1. Actin stress fibres were visualized as described in Methods. Representative examples of stress fibres in individual cells are shown.