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(A) Original tracing illustrating the effects of Y-27632 on the ET-1-induced increase in [Ca2+]i in CHO-ETBR. The cells loaded with fluo-3 were incubated with 10 μM Y-27632 for 15 min before 10 nM ET-1 stimulation. (B, C) Effects of 10 μM Y-27632 on 10 nM ET-1-induced transient (B) and sustained (C) increase in [Ca2+]i in CHO-ETBR. The experimental protocols were described in Methods. Data are presented as mean±s.e.m. of three experiments.
