Figure 2.

Characterization in inside-out configuration of the SK_ap-ins channel. (A) Current-voltage relationship obtained in inside-out configuration in asymmetrical K⁺ concentration, pipette solution containing 5.6 mM K⁺ and bath solution containing 130 mM K⁺. Each point is the mean±s.e.mean of 13 experiments. (B) Effect of various intracellular [Ca²⁺] on the normalized open state probability (P_o/P_omax), at holding potential of 0 mV. Values are given as mean±s.e.mean for 3–7 experiments. Data were fitted with a Boltzmann equation and EC₅₀ was 795 nM [Ca²⁺]_i.