Figure 3.

Effect of auranofin on mitochondrial oxygen uptake. Rat liver mitochondria (RLM, 1 mg ml⁻¹) were incubated in 0.1 M sucrose, 50 mM KCl, 1 mM MgCl₂, 1 mM NaH₂PO₄, 20 mM HEPES (pH 7.4), 7 mM succinate, 0.3 mM ADP and, when indicated, 2 μM auranofin. In (C), 0.25 mM EGTA was also present.