Figure 1.

Concentration–contraction curves with (A) ET-1, (B) S6c and (C) 5-CT as agonists. The effect of co-culturing with staurosporine, RO 31-7549 and H 89, respectively, is displayed. Data are displayed as mean±s.e.mean, n indicates number of rats in each experiment. The data for the curves displaying results after co-culture with staurosporine have been corrected due to lower K⁺ contractility (see Methods). Staurosporine was in general capable of inhibiting changes approximating the concentration–response curves to the curves derived from experiments conducted on fresh arterial segments. Ro 31-7549 effectively inhibited the appearance of a contractile ET_B response, and H 89 had in general an augmenting effect on the changes induced by organ culture. For more details see Table 1 and the results section.