Figure 2.
Effect of antibody R73 on total T cells (A) and eosinophils (B) recovered from lung tissue after antigen challenge in the Brown Norway rat. Antigen (ovalbumin)-sensitized Brown Norway rats were either unchallenged and untreated (open bars), exposed to inhaled saline and untreated (shaded bars) or challenged with inhaled antigen aerosol (solid bars) and either untreated, given antibody R73 (300 μg kg−1, i.v.) or control antibody (mouse IgG1). 24 h later, cells were isolated from lung tissue by enzymatic disaggregation. Cells were counted by flow cytometry (a) or by light microscopy (b). Group size was 8. Results represent mean±s.e.mean. †P<0.05 compared with saline challenged group; *P<0.05 compared with challenged, control antibody treated group.