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Changes in expression of mRNA for cytokines and chemokines in lung tissue 8 h after antigen challenge in the Brown Norway rat. Agarose gels of PCR-amplified cDNA prepared by reverse transcription of RNA extracted from lung tissue. Antigen (ovalbumin)-sensitized Brown Norway rats were either unchallenged, exposed to inhaled saline (sham challenged) or challenged with inhaled antigen aerosol.
