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. 2002 Oct 30;137(6):892–900. doi: 10.1038/sj.bjp.0704873

Figure 1.

Figure 1

Identification of the A2690C single nucleotide polymorphism resulting in the K897T exchange in the patient's HERG protein. (A) Electropherograms from sequencing analyses of genomic DNA (left and middle panel) indicate heterogeneity for the position 897 in the HERG protein. An A–C substitution is observed at position 2960 (K897T) in the coding sequence. Right panel shows the variant sequence of a subcloned PCR fragment. (B) Topological model of the HERG protein, showing the location of the mutation K897T within the intracellular C-terminus, 41 amino acids downstream of the putative cyclic nucleotide binding domain (CNB). (C) Protein sequence alignment of members of the eag potassium channel family (human and mouse eag channels, and human, rat and mouse ERG channels), showing conservation of the lysine at codon 897 (HERG numbering, indicated by arrow). GCG software (Wisconsin Package, Version 10.1) was used.