Figure 3.
Two-electrode voltage clamp records of wild-type and K897T HERG and the respective IKr currents expressed in Xenopus oocytes. (A–D), Representative current traces from oocytes expressing the indicated proteins using protocol (3). In (C) and (D) deactivation kinetics are faster as compared to homomeric expression of the HERG subunit in (A) and (B) respectively. (E) Current voltage relationships from (A) and (C) showing the influence of MiRP1 on activation of wild-type HERG channels. (F) Current voltage relationships from (B) and (D) showing the influence of MiRP1 on activation of HERG K897T channels. Tail current amplitude was measured at the maximum of the current trace, squares represent values from homomeric HERG expression, triangles represent values from coexpression with the MiRP1 subunit; numbers of experiments as indicated in Table 1.