Figure 3.

Flow cytometric analysis quantification of apoptosis in H9c2 cardiac myocytes exposed to different concentrations of CsA for 24 h, CsA combined with 100 μM H₂O₂ for 24 h, or pre-treated with CsA for 24 h before 100 μM H₂O₂ treatment for another 24 h. Cells were examined for cell apoptosis with the annexin-V/PI staining technique as described in Materials and methods. Data are expressed as mean±s.e.mean (n = 5). *P<0.05, as compared to the cells treated with 100 μM H₂O₂; ¶P<0.05, as compared to the untreated cells.