Figure 7.

Effects of M₂ and EGFP RNA_i on CCh-induced bradycardia in zebrafish embryos. Each one-cell stage embryos was injected with M₂ or EGFP dsRNA, and then incubated at 28.5°C until the heart rate was measured at room temperature. The same embryo was assayed for CCh-induced bradycardia at 56, 72 and 96 h.p.f. CCh-induced bradycardia was performed as described in Methods. The ratio of the heart rate is defined as in Figure 6 legend. The data represent the mean and the s.e.mean for three independent experiments, each with 8–10 individuals. A difference between the M₂ dsRNA and the wild-type, or EGFP was considered significant with P<0.05 (*,⁺) or P<0.01 (**,⁺⁺) using Student's t-test.