Figure 4.

Effect of SP, NKA and NKB on the [Ca²⁺]_i and tension in the rabbit corpus cavernosum strips without an endothelium. Representative recordings showing the effect of 1 μM SP, NKA and NKB on the [Ca²⁺]_i (upper trace) and tension (lower trace) of the rabbit corpus cavernosum in normal PSS (a, c, e) and in Ca²⁺-free PSS containing 0.3 mM EGTA (b, d, f). Ca²⁺-free PSS with 0.3 mM EGTA was applied 3 min before and during the addition of SP, NKA and NKB. The developed tension and [Ca²⁺]_i were expressed as a percentage, assigning the 10 μM phenylephrine-induced contraction to be 100%. The dots represent the wash out with PSS.