Figure 6.

Effect of P-CTX-1b on IP₃ mass levels in rat myotubes. Confluent plates of rat myotubes were washed three times with PBS, and incubated for the times indicated with 10 nM P-CTX-1b. The mass of IP₃ in the extract once neutralized was measured by a radioligand receptor assay, as described in Methods. (Inset) Plates of rat myotubes were rinsed with PBS and incubated with 10 nM P-CTX-1b for 15 s in the absence or in the presence of 1 μM TTX. Each column represents the mean value±s.e.mean obtained from three to six different experiments done in triplicate. (*) denotes P<0.0001 Student's t-test.