Figure 1.

Effect of the protein synthesis inhibitor, actinomycin D, on IL-1β- or TNFα-induced leukocyte adhesion and transmigration in mouse cremasteric venules. Mice were injected intrascrotally with saline (400 μl), IL-1β (30 ng in 400 μl saline) or TNFα (300 ng in 400 μl saline) with or without actinomycin D (0.2 mg kg⁻¹) and leukocyte firm adhesion (a) and transmigration (b), per 100 μm vessel segment, were quantified by intravital microscopy 4 h later, as detailed in Methods. Results are presented as mean±s.e.mean for n=5 mice per group. Statistically significant differences (one-way ANOVA and Newman-Keuls post-test) between control and drug-treated groups are shown by asterisks, *P<0.05 and ***P<0.001.