Figure 4.

The effect of the LTB₄ receptor antagonist, CP-105,696, on IL-1β- and TNFα-induced leukocyte adhesion and transmigration in mouse cremasteric venules. Mice were pretreated with vehicle or CP-105,696 (100 mg kg⁻¹, p.o.) 19 h prior to intrascrotal administration of saline (400 μl), IL-1β (30 ng in 400 μl saline) or TNFα (300 ng in 400 μl saline). Four hours later, leukocyte responses of adhesion (a) and transmigration (b), per 100 μm vessel segment, were quantified by intravital microscopy, as detailed in Methods. Values represent mean±s.e.mean of n=5 mice per group. Statistically significant differences (one-way ANOVA and Newman-Keuls post-test) between vehicle and drug-treated groups are shown by asterisks, *P<0.05 and ***P<0.001.