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. 2002 Nov 27;137(8):1355–1361. doi: 10.1038/sj.bjp.0704998

Figure 4.

Figure 4

Interaction of Ac-RYYRIK-NH2 with nociceptin/orphanin FQ (N/OFQ) (A) and of naloxone benzoylhydrazone (NalBzOH) with Ac-RYYRIK-NH2 (B) in superfused mouse brain cortex slices preincubated with [3H]-noradrenaline ([3H]-NA). The medium contained desipramine 1 μM, rauwolscine 1 μM and naloxone 10 μM. In panel (A), Ac-RYYRIK-NH2 was present in the medium throughout superfusion whereas N/OFQ was added from 62 min onward. In panel (B), NalBzOH was present in the medium throughout superfusion whereas Ac-RYYRIK-NH2 was added from 62 min onward. Two periods of electrical stimulation (0.3 Hz) were administered, after 40 (S1) and 90 min (S2) of superfusion, and the ratio of the overflow evoked by S2 over that evoked by S1 was determined. Tritium overflow is given as per cent of the S2/S1 value in the corresponding controls (not shown). Means±s.e.mean of 4–6 experiments. *P<0.05, **P<0.001.