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MTSM binds at L338C but does not inhibit currents. MTSM applied to the L338C receptor does not inhibit the current evoked by ATP (30 μM, 2 s, bars above traces), but it prevents the effect of a subsequent application of MTSEA, MTSET and MTSES do not inhibit the current themselves, and also do not block the inhibition by subsequent MTSEA. All MTS compounds applied at 1 mM for 8 min, as indicated by bars. Similar results were observed for at least three cells in each case.
