Figure 7.

The effects of nifedipine, bisindolymaleimide and prazosin upon phenylephrine-stimulated increases in intracellular Ca²⁺ ([Ca²⁺]_i) in human cultured prostatic stromal cells. Cells were incubated with the FURA-2AM (5 μM) prior to the addition of phenylephrine. Antagonists and blockers were added 45–60 min prior to the addition of phenylephrine. (a), (b) and (c) Show mean changes in [Ca²⁺]_i over 30 min, in response to phenylephrine (10 μM), in the presence and absence of prazosin (1 μM), bisindolymaleimide (1 μM) and nifedipine (10 μM) respectively. ^*Significant when compared to phenylephrine (10 μM). P<0.05, Dunnett's test (n=6).