Figure 4.

Effect of carbachol on the evoked overflow of tritium from atria (A), bladder (B) and vas deferens (C) of M₄-wildtype or M₄-knockout mice. After preincubation with ³H-noradrenaline tissues were superfused and stimulated electrically six times by 20 pulses/50 Hz (S₁ to S₆). Carbachol was added at increasing concentrations (abscissae) before S₂ to S₆. Ordinates, evoked overflow of tritium, calculated from S_n/S₁ ratios and expressed as a percentage of the corresponding control (no carbachol). Means±s.e.mean from n=5 to 16 tissue pieces. Significant differences from corresponding control (no carbachol): *P<0.05. Significant differences from M₄-wildtype: #P<0.05.