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. 2003 Feb 5;138(3):481–493. doi: 10.1038/sj.bjp.0705055

Figure 4.

Figure 4

S1P5 receptor expression in CHO-K1 cells causes cell rounding. CHO cells transiently transfected with S1P5 or pcDNA3.1(+) vector and GFP (4 : 1) cDNA were grown for 34 h in Iscove supplemented with FBS, csFBS, or csFBS+1 μM S1P. Plates were then analysed by fluorescence microscopy. The human S1P5 receptor induced cell rounding that was more pronounced in transfected cells cultivated in medium containing 10% FBS as compared with medium containing csFBS. Addition of 1 μM S1P to csFBS containing medium led to a complete round morphology of S1P5 transfected cells. Vector transfected cells displayed the normal CHO cell morphology.