Figure 8.
S1P-mediated internalization of a HA-tagged wild-type S1P5 receptor. COS-7 cells transiently expressing the HA-tagged S1P5 receptor were grown and transfected in 48-well plates. Seventy-two hours after transfection cells were fixed and surface expressed receptors detected with a surface ELISA as described in detail in the Method section. S1P concentration-dependently internalizes a certain percentage of S1P5 receptors. Data are shown as means±s.e.mean of three independent experiments performed in quadruplicates. *P<0.05, **P<0.01, ***P<0.001 indicate significant differences of the respective multiple comparisons.