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Typical time-course of LTD4-induced [Ca2+]i increase in isolated rat aortic smooth muscle cells in the absence of extracellular calcium (200 μM EDTA). LTD4 (10 nM) was administered at time=0 and maintained in the perfusion chamber until the end of experiment as indicated. Curves are the mean of at least 11 single cells. In each point, standard errors (s.e.mean) do not exceed 10% of values.
