Figure 5.

Ioxaglate produces a concentration-dependent increase in the release of nafamostat mesilate-sensitive tryptase-like protease from rat peritoneal mast cells. Tryptase activity was assessed by the degradation of a synthetic tryptase substrate t-Boc-Phe-Ser-Arg-MCA to methylcoumarine, and its release was expressed as the percentage of activity in the incubation medium to the total. The tryptase-like activity was determined in the presence of 10 nM nafamostat mesilate (Nafam). Each column represents the mean±s.e.mean for n=4–6 experiments. ^**P<0.01 versus control, ^††P<0.01 versus ioxaglate (100 mg iodine ml⁻¹) alone.