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. 2003 Mar 7;138(5):921–931. doi: 10.1038/sj.bjp.0705127

Figure 2.

Figure 2

Chronic 300 nM ADR, 30 μM NA, and 1 μM ISO desensitize the β-AR response in BE(2)-C cells. BE(2)-C cells were incubated for 16–24 h with vehicle (0.1 mM ascorbate), 300 nM ADR, 1 μM NA, 30 μM NA or 1 μM ISO. After washing, intact cells were assessed for ISO-stimulated (250 nM) cAMP accumulation. Chronic 300 nM ADR, 30 μM NA, and 1 μM ISO (n=2–9; *P<0.05), but not 1 μM NA (n=6), pretreatment desensitized the β-AR response to ISO compared to the corresponding vehicle-treated control. None of the pretreatments altered basal cAMP levels (27.6±6.1 pmol mg−1 protein). Data represent mean±s.e.mean; comparisons were made by ANOVA with Dunnett's post-hoc test.