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Detection of apoptosis of progenitor cells induced by theophylline plus growth factors (GM-CSF, IL-3, SCF and IL-5) using PI staining by flow cytometric analysis. Progenitor cells from normal subjects (a) or patients with asthma (b) were treated with various concentrations of theophylline, collected after 72-h culture period and stained with PI to detect DNA fragmentation by flow cytometry. Percentages indicate the percentage of apoptotic cell nuclei/total cell nuclei.
