Figure 5.

Direct immunofluorescence flow cytometry was performed to detect intracellular expression of Bcl-2 on CD34+ cells. The mean fluorescence intensity (MFI) of Bcl-2 expression in cells treated with (solid bars) or without (hatched bars) 5 μg ml⁻¹ theophylline (a) or 0.1 mM db-cAMP (b) or 0.1 mM rolipram (c) or 1 μM salbutamol (d) for 24 h in normal subjects (normal, n=7) or patients with mild intermittent asthma (asthma, n=8). ^*P<0.05 compared with the progenitor cells from normal subject in the absence of treatment. ^**P<0.05 compared with the progenitor cells in the absence of treatment. Data are means±s.e.m.