Figure 1.

Aspirin suppresses B16 cellular proliferation. (a) B16 cells (at 4 × 10³ well⁻¹) were seeded in 96-well plates and allowed to adhere overnight. Aspirin at the indicated concentrations was added for 1 day. Optical density representing viable cells was determined by the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay. (b) B16 cells (at 4 × 10⁵ well⁻¹) were seeded in six-well plates and allowed to adhere overnight. Aspirin at the indicated concentrations was added for 1 day. The number of viable cells was determined by microscopic cell counts. Inhibition of proliferation is calculated as percentage of control cultures that were not treated with aspirin, mean±s.d. The inhibition was statistically significant at ^*P<0.025, ^**P<0.01, ^***P<0.005 for panel a, and ^*P<0.025, ^**P<0.005, ^***P<0.0005 for panel b; n=3.