Figure 3.
The JNK and p38 inhibitor SB202190 decreases aspirin-induced suppression of B16 cellular proliferation. B16 cells (at 4 × 103 well−1) were seeded in 96-well plates and allowed to adhere overnight. Aspirin at 3 mMwas added for 1 day. SB202190 was added at the indicated concentrations, 30 min before the addition of aspirin, and was removed 2.5 h afterwards. Optical density representing viable cells was determined by the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay. Inhibition of proliferation is calculated as percentage of control cultures that were not treated with aspirin, mean±s.d. The effect of SB202190 (in comparison with None=only aspirin) was statistically significant at *P<0.05, **P<0.005, ***P<0.0005; n=3.