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Effect of prolonged exposure to aspirin on B16 proliferation. B16 cells (at 4 × 103 well−1) were seeded in 96-well plates and allowed to adhere overnight. Aspirin at 3 mMwas added for 1 and 3 days. Optical density representing viable cells was determined by the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay. Cellular proliferation is calculated as percentage of control cultures that were not treated with aspirin (defined as 100%), mean±s.d; n=3.
