Figure 6.
Cytotoxic effect of aspirin and BCNU towards B16 cells. B16 cells (at 4 × 103 well−1) were seeded in 96-well plates and allowed to adhere overnight. Aspirin at 1 and 3 mM, and/or BCNU at 50 μM, were added for 1 day. Optical density representing viable cells was determined by the CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay. Cytotoxicity is calculated as percentage of control cultures that were not treated, mean±s.d., n=3. The synergistic effect (the difference between cytotoxicity in the presence of both drugs together, and the sum of the cytotoxicities of each drug administered separately) was significant at P<0.05, for each combination of aspirin and BCNU.