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Nucleotide-induced IL-8 production is potentiated by GM-CSF. Cells were incubated for 30 min in the presence or absence of 10 ng ml−1 GM-CSF. Eosinophils were then stimulated with 1 mM of the indicated nucleotides and cultured for 24 h. Supernatants were then collected and IL-8 content was determined by ELISA. Data are expressed as mean±s.e.m. (n=4).
