Table 3.
Effects of L-NAME and K+ channel blockers on relaxation to abn-cbd in endothelium-intact small mesenteric arteries precontracted with methoxamine
| Abn-cbd | pEC50 | Emax (%) | n |
|---|---|---|---|
| Control | 6.0±0.1 | 93±2 | 9 |
| +L-NAME | 5.9±0.1 | 97±1 | 4 |
| +L-NAME+apamin+charybdotoxin | 5.4±0.1 | 79±10 | 6* |
| Control | 5.8±0.1 | 95±2 | 5 |
| +L-NAME+apamin | 5.9±0.2 | 78±6 | 4* |
| +L-NAME+apamin+iberiotoxin | 5.5±0.1 | 85±6 | 4* |
| Control | 5.8±0.1 | 89±2 | 5 |
| +L-NAME+iberiotoxin | 5.8±0.1 | 88±4 | 6 |
| +L-NAME+charybdotoxin | 5.6±0.1 | 96±3 | 4 |
All experiments were performed in the presence of 10 μM indomethacin. Data are expressed as mean±s.e. mean. pEC50 and Emax values were obtained directly from individual log concentration – response curves. n represents the number of animals. The inhibitors were used at the following concentrations: L-NAME (300 μM), apamin (50 nM), charybdotoxin (50 nM) and iberiotoxin (50 nM).
*
Indicates significantly different from control values (two-way analysis of variance; P<0.01).