Figure 7.

Concentration-dependent increases in c-Jun N-terminal kinase (JNK) activity stimulated by MCh in CHO-m3 and -m2m3 cells. Confluent monolayers of CHO cells were stimulated with the indicated concentrations of MCh for 30 min. Incubations were then terminated, cell lysates prepared and JNK assays performed as described in the Methods section. Data are expressed as enzymic activities (expressed as fmol phosphate incorporated into c-Jun fusion protein per min per mg of cell protein) and represent means±s.e. mean for four (CHO-m3) or six (CHO-m2m3) separate experiments. The inset shows a representative experiment to illustrate a typical time-course of MCh (100 μM)-stimulated JNK activation in the two cell-lines.