Table 1.
Assessment of receptor expression levels in CHO-m2, -m3 and -m2m3 cell-lines by [3H]-NMS saturation binding and quantitation of M2 and M3 mACh receptor subpopulations in B2 and B7 clones
| Cell-line | Bmax (pmol mg−1 protein) | Ratio M2 : M3 | No. expts. (n) |
|---|---|---|---|
| CHO-m2 | 0.86±0.05 | 100 : 0 | 4 |
| CHO-m3 | 1.48±0.12 | 0 : 100 | 4 |
| CHO-m2m3 (B2) | 2.16±0.21 | 27 : 73 | 6 |
| CHO-m2m3 (B7) | 2.28±0.26 | 30 : 70 | 3 |
Bmax values are expressed as means±s.e. mean for the indicated number (n) of experiments. M2 : M3 ratios were determined by two-site analysis (GraphPad Prism) of tripitramine displacement isotherms for a single concentration of [3H]-NMS (approx 0.8 nM).