Figure 6.

Inhibition of transient Ca²⁺ increase by different inhibitors of signal transducing elements. Values represent maximal fluorescence change stimulated by VV-H-7 (10 μM), expressed relative to the maximum VV-H-7 (10 μM), response and are the means±s.e.mean from at least eight independent experiments. (a) Specific inhibitors of the Ca²⁺/ATPase: cyclopiazonic acid (10 μM) and thapsigargin (1 μM), the IP₃-receptor-antagonist xestospongin C (20 μM) and the PLC-inhibitor U 73122 (3 μM) were incubated for 20 min prior to VV-H-7 application. (b) Phosphoinositid-3-kinase inhibitor LY 294002 (3 μM) and inhibitor of the PKC: Gö 6850 (3 μM) and Chelerythrine (3 μM) were incubated for 20 min prior to VV-H-7 application.