Figure 4.

Effect of cacospongionolide B on NF-κB-DNA binding in nuclear extracts of mouse peritoneal macrophages. (a) Intact cell treatment. Cells were preincubated with cacospongionolide B for 30 min followed by zymosan stimulation for 2 h and then nuclear extracts were obtained as described in Methods. (b) Nuclear extract treatment. Cells were stimulated with zymosan for 2 h and then nuclear extracts were obtained as described in Methods. B: basal (nonstimulated cells); Z: zymosan. Cacospongionolide B (CB) or 15-deoxy-Δ^12,14 -PGJ₂ (PGJ₂) was incubated with nuclear extracts for 10 min. The figures are representative of three similar experiments.