Figure 6.

Translocation of PKCα induced by treatment with 12-deoxyphorbol 13-isobutyrate in pregnant myometrium. (a) Myometrial strips from day 21 pregnant rats were stimulated with 40 mM K⁺ and 10⁻⁶ M 12-deoxyphorbol 13-isobutyrate (10⁻⁶ M, DPB), for different times (5 and 30 min). (b) Statistical results for the cytosolic and membrane fractions were obtained from three independent experiments. The level of PKCα in the quiescent state was defined as 100%. ^*, # Denote significant differences from the results of the cytosol and the membrane in the quiescent states, respectively (P<0.05).