Figure 5.

RT–PCR analysis of α_2Aadrenoceptor (α_2A-AR) and β-actin mRNA expression in mucosal (Muc) and muscular (Musc) layers of ileum or colon isolated from animals in the absence (vehicle) or in the presence of DNBS-induced colitis (DNBS). Each panel displays two representative agarose gels, referring to the amplification of α_2A-adrenoceptor and β-actin cDNAs, and a column graph referring to the densitometric analysis of α_2A-adrenoceptor cDNA bands normalized to the expression of β-actin. M=size markers. Each column represents the mean of five separate experiments±s.e.m. (vertical bars). Significant difference from the respective values obtained in vehicle-treated animals: ^*P<0.05.