. 2003 May 29;139(2):309–320. doi: 10.1038/sj.bjp.0705249

Table 3.

Effects of UK-14,304, rauwolscine or phentolamine on tritium overflow

	Evoked tritium overflow
	S₁	S₂/S₁
Vehicle control	1.72±0.08	0.96±0.04
UK-14,304 1 μM	1.68±0.06	0.52±0.03^a
Rauwolscine 1 μM	1.65±0.09	1.68±0.18^a
Rauwolscine 10 μM	1.76±0.09	2.21±0.23^a
Phentolamine 1 μM	1.73±0.07	1.97±0.15^a
Phentolamine 10 μM	1.70±0.05	2.84±0.17^a
DNBS control	1.07±0.09^*	0.99±0.03
UK-14,304 1 μM	1.03±0.07^*	0.35±0.06^*,^a
Rauwolscine 1 μM	1.02±0.05^*	2.06±0.11^*,^a
Rauwolscine 10 μM	1.05±0.05^*	2.73±0.13^*,^a
Phentolamine 1 μM	1.03±0.08^*	2.92±0.15^*,^a
Phentolamine 10 μM	1.11±0.06^*	3.66±0.11^*,^a

Longitudinal muscle strips of ileum were obtained from rats either in the absence or in the presence of DNBS-induced colitis. Each preparation was preincubated with [³H]noradrenaline, superfused with Krebs solution, and subjected twice to electrical stimulation (0.5 ms, 20 V cm⁻¹, 8 Hz, 480 pulses). The effects of test drugs on tritium outflow are expressed as S₁ (percentage of the tritium content of the tissue at the onset of the first electrical stimulation), or S₂/S₁ (ratio of the percentage release during the second stimulation over that obtained during the first stimulation). Each value represents the mean of five to six experiments±s.e.m.

Significant difference from the respective values obtained from vehicle-treated rats: ^*P<0.05

significant difference from the respective control values: ^aP<0.05.