Figure 1.

Ang1 prevents permeability increases across endothelial monolayers. (a) BLMVEC monolayers were exposed to 250 ng ml⁻¹ Ang1 or solvent for 2 h before adding 1 μM bradykinin for 1 h, 100 μM histamine for 30 min, or 1 U ml^–1 thrombin for 1 h. Values are means+s.e.m., n=3; ^*P<0.05 from vehicle. The passage of BSA under baseline conditions was of 31.5±5 μg monolayer⁻¹ (30 min)^–1. (b) EA.hy 926 monolayers were exposed to 250 ng ml⁻¹ Ang1 or solvent for 1 h before adding 10 nM PAF, 1 μM bradykinin, 100 μM histamine or 0.01 U ml⁻¹ thrombin for 45 min. Values are means±s.e.m., n=6–12; ^*P<0.05 from vehicle. The passage of HRP under baseline conditions was 16.4±1.7 ng HRP monolayer⁻¹ (30 min)⁻¹.