Figure 2.

Ang1 decreases neutrophil adherence stimulated by thrombin or H₂O₂. Confluent EA.hy 926 cells were preincubated with 250 ng ml⁻¹ Ang1 or solvent for 1 h before treatment with 50 nM PAF for an additional hour, 1 ng ml⁻¹ hTNFα for 4 h 30 min, 0.005 U ml⁻¹ thrombin for 1 h, 0.5 mM H₂O₂ for 1 h or 10 μM histamine for 1 h. PMN were then added for 30 min. Values are means±s.e.m., n=3–4; ^*P<0.05 from vehicle.