Table 2.
Carbachol EC50 (M) in amylase release and IP production in the absence and the presence of the antagonists of the M2, M4 and nicotinic receptors
| Additions | Control | Castrated | ||
|---|---|---|---|---|
| Amylase | IP | Amylase | IP | |
| None | 1.54 × 10−5 | 1.78 × 10−5 | 9.88 × 10−7 | 7.94 × 10−7 |
| AF-DX 116 | 1.58 × 10−5 | 2.19 × 10−5 | 7.40 × 10−7 | 7.20 × 10−7 |
| Tropicamide | 1.41 × 10−5 | 1.66 × 10−5 | 7.90 × 10−7 | 8.10 × 10−7 |
| Benzoquinonium | 1.35 × 10−5 | 2.00 × 10−5 | 8.50 × 10−7 | 7.94 × 10−7 |
Parotid slices were preincubated in the absence or presence of 1 × 10−5 M of the antagonists added at the beginning of the incubation time, followed by determination of carbachol dose-dependent stimulation of amylase release or IP production as described in Methods. Results shown are one of four experiments with similar results.