Figure 5.
Loperamide has no direct effect on the large single-channel currents measured in excised patches from HIT cells. Recording from an inside-out patch configuration before (panel a) and after (panel b) addition of 30 μM loperamide to the solution superfusing the cytoplasmic side of the cell membrane. Insets show details on an expanded time scale. The graphs in panel c illustrate the mean open time (left), open probability (middle), or amplitude (right) of the single-channel current before (control) and after (LPM) addition of loperamide. Vertical bars show s.e.m., n=3. (Holding potential=0 mV; all solutions were Ca2+ free and contained 5 mM EGTA; records are representative of five similar experiments conducted in different cells.)