Figure 6.

Carbachol increased the open probability of the loperamide-stimulated channel in HIT cells. Recordings under control conditions with 2.5 mM Ca²⁺ (panel a), when the cell was exposed to 60 μM loperamide in Ca²⁺-free solution (panel b) and when 100 μM carbachol was also added to the loperamide-containing but Ca²⁺-free solution (panel c). (Recording in the cell-attached configuration; holding potential=0 mV; glucose concentration was 2.8 mM; records are representative of three similar experiments conducted in different cells.)