Figure 1.

Effect of LTs on human neutrophil apoptosis. Neutrophils (5 × 10⁶ cells ml⁻¹) were cultured in serum-supplemented Iscove's MDM alone (control) or in identical medium containing stimuli (final concentration). (a) LTB₄ (1 μM), LTC₄ (1 μM), LTD₄ (1 μM), GM-CSF (50 U ml⁻¹) or LPS (1 μg ml⁻¹) and (b) LTB₄ (1 nM–1 μM). Cells were harvested following 20 h in culture and assessed for morphological features of apoptosis. Data represent the means±s.e.m. of three separate experiments, each performed in triplicate, where ^* represents P<0.05 compared to control values.