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. 2003 May 29;139(2):388–398. doi: 10.1038/sj.bjp.0705265

Figure 2.

Figure 2

Measurement of cytosolic calcium levels in freshly isolated human neutrophils in response to agonist stimulation. Cytosolic calcium measurements (nM) in fura-2-loaded, neutrophils (2 × 106 cells ml−1) over 350 s. Buffer alone (control, a,c,e) or CP-105,696 (1 μM; b,d,f) was added to the cell suspension followed by the sequential addition of PAF (100 nM; a, b), LTB4 (100 nM; c, d), or fMLP (100 nM; e, f), after 245 s. The data show traces obtained from a typical experiment performed at least three times.